›› 2011, Vol. 23 ›› Issue (6): 465-467.doi: 10.3969/j.issn.1004-616x.2011.06.015

• 核辐射安全专家论坛 • 上一篇    下一篇

hprt基因突变对宫颈癌放疗损伤的评估

武丽蕊,王兰朋,李红霞1,王德华,孙 莎   

  1. 1. 中国石油天然气集团公司中心医院妇产科，河北 廊坊 065000；2. 廊坊市人民医院胸外科，河北 廊坊 065000；3. 天津医科大学总医院妇产科，天津 300070
  • 收稿日期:2011-06-18 修回日期:2011-09-27 出版日期:2011-11-30 发布日期:2011-11-30
  • 通讯作者: 武丽蕊

Assessment of radiotherapy damage using hprt gene mutation in cervical cancer patients

WU Li-rui，WANG Lan-peng ，LI Hong-xia，WANG De-hua，SUN Sha   

  1. 1. Department of Gynecology and Obstetrics, China National Petroleum Company Central Hospital, Langfang 065000, Hebei; 2. Department of the Thoracic Surgery, The People’s Hospital Lang Fang, Langfang 065000, Hebei; 3. Department of Gynecology and Obstetrics, General Hospital of Tianjin Medical University, Tianjin 300070, China
  • Received:2011-06-18 Revised:2011-09-27 Online:2011-11-30 Published:2011-11-30
  • Contact: WU Li-rui

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摘要： 目的： 探讨hprt基因突变对宫颈癌放疗损伤的评估作用。方法：取15例宫颈鳞癌放疗患者放疗前及放疗累积剂量为10～60 Gy时的外周静脉血，采用多核细胞法检测hprt基因突变率。结果：在照射累积剂量为30、40和50 Gy时，hprt基因突变率在照射后升高，与照射前相比，差别有统计学意义 (P＜0.05)。当累积剂量达60 Gy 时，hprt基因突变率虽然较照射前升高，但差别无统计学意义 (P＞0.05)。在0～40 Gy，hprt基因突变率基本呈线形上升，且突变率与累积剂量间呈现较好的剂量-效应关系 (r＝0.900，P＜0.05)。回归方程为y＝0.459＋0.014 x。结论：在累积剂量0～40 Gy范围内，hprt基因突变率在放疗后明显升高，呈现良好的剂量-效应关系，可用于评价放疗造成的损伤，是具有应用潜质的生物剂量计。

关键词: 宫颈癌, hprt基因位点突变, 放疗损伤, 剂量-效应关系

Abstract: OBJECTIVE: To assess the radiotherapy damage using hprt gene mutation in cervical cancer patients. METHOD：Blood samples were collected from 15 cervical cancer patients exposed to radiation at the accumulated dose of 0-60 Gy. hprt gene mutation was studied by multinucleated cell assay. RESULT：Mutation frequency of hprt gene increased after radiation. The frequency increased significantly at dose 30，40 and 50 Gy compared to 0 Gy (P＜0.05 ). At the accumulated dose of 60 Gy，the mutation frequency was also higher than 0 Gy，but not statistically significant. The increase of hprt gene mutation frequency was linear from 0-40 Gy. There was a good dose-response effect between mutation frequency of hprt gene and accumulated dose ( r＝0.900，P＜0.05 ). Analysis showed a linear dose-response with an equation of y＝0.459＋0.014 x ( r＝0.900， P＜0.05). CONCLUSION：Mutation frequency of hprt gene increased after radiation from 0-40 Gy. There was a good dose-response relationship between mutation frequency and accumulated dose. Mutation frequency of hprt gene could be a valuable biodosimeter.

Key words: cervical cancer, hprt gene mutation, radiation-induced damage, dose-response relationship

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